Mannheimia haemolytica & Pasteurella multocida
- M. haemolytica is the number one cause of bacterial pneumonia
- Newport uses leukotoxin assay to aid in isolate selection of M. haemolytica
- A dual fermentation manufacturing process is utilized to enhance leukotoxin and IROMP (Iron Regulated Outer Membrane Protein) expression in the product
Serological Response to Vaccination Using a Multivalent Autogenous Respiratory Bacterin
Both bovine leukotoxin and iron regulated outer membrane proteins (IROMPs) have been recognized as important antigens of Mannheimia haemolytica. Newport Laboratories has developed a unique dual fermentation process using specialized growth media to maximize the levels of both of these antigens in the manufacture of autogenous bacterins. Assays of final harvest fluids of representative autogenous bacterins produced by Newport Laboratories have confirmed increased levels of leukotoxoid and IROMPs compared to conventional manufacturing process.
Enhancing Leukotoxin and IROMP Levels in the Production of Autogenous Bacterins
Bovine pasteurellosis caused by Mannheimia haemolytica, primarily due to serotypes A1, A6 and A9, results in a severe fibrinous pneumonia. Several virulence factors have been identified with this organism, including cytotoxic leukotoxin and outer membrane proteins. Some virulence factors, specifically outer membrane proteins, are considered to be cross-reacting and capable of cross-protection, but some appear to be serotype specific.